Offense
| # | Name | Pos | Ht | Wt | Exp | College | Status |
| 63 | Tyler Biadasz | C | 6'3" | 316 lbs | 6 | Wisconsin | Reserve |
| 61 | Julian Good-Jones | G | 6'5" | 313 lbs | 2 | Iowa State | Active |
| 67 | Nick Allegretti | G | 6'4" | 310 lbs | 7 | Illinois | Active |
| 71 | Andrew Wylie | G | 6'5" | 304 lbs | 8 | Eastern Michigan | Active |
| 75 | Chris Paul | G | 6'4" | 324 lbs | 4 | Tulsa | Active |
| 76 | Sam Cosmi | G | 6'6" | 309 lbs | 5 | Texas | Reserve |
| 53 | Lucas Niang | OT | 6'6" | 315 lbs | 4 | TCU | Reserve |
| 72 | Josh Conerly Jr. | OT | 6'4" | 315 lbs | R | Oregon | Active |
| 74 | Brandon Coleman | OT | 6'6" | 320 lbs | 2 | TCU | Active |
| 77 | George Fant | OT | 6'5" | 296 lbs | 10 | Western Kentucky | Active |
| 78 | Laremy Tunsil | OT | 6'5" | 313 lbs | 10 | Mississippi | Active |
| — | Malaesala Aumavae-Laulu | OT | 6'5" | 344 lbs | 3 | Oregon | Practice Squad |
| 68 | Timothy McKay | OT | 6'4" | 312 lbs | R | North Carolina | Practice Squad |
| 73 | Trent Scott | OT | 6'5" | 320 lbs | 8 | Grambling State | Active |
| 5 | Jayden Daniels | QB | 6'4" | 210 lbs | 2 | LSU | Active |
| — | Jeff Driskel | QB | 6'4" | 231 lbs | 9 | Louisiana Tech | Active |
| 8 | Marcus Mariota | QB | 6'4" | 222 lbs | 11 | Oregon | Active |
| 14 | Josh Johnson | QB | 6'3" | 214 lbs | 11 | San Diego | Active |
| 15 | Sam Hartman | QB | 6'1" | 209 lbs | 1 | Notre Dame | Active |
| 30 | Austin Ekeler | RB | 5'10" | 200 lbs | 9 | Western State | Reserve |
| — | Chase Edmonds | RB | 5'9" | 201 lbs | 8 | Fordham | Active |
| 22 | Jacory Croskey-Merritt | RB | 5'11" | 208 lbs | R | Arizona | Active |
| 26 | Jeremy McNichols | RB | 5'9" | 205 lbs | 5 | Boise State | Active |
| 36 | Chris Rodriguez Jr. | RB | 5'11" | 224 lbs | 3 | Kentucky | Active |
| 82 | Ben Sinnott | TE | 6'4" | 245 lbs | 2 | Kansas State | Active |
| — | Gee Scott Jr. | TE | 6'3" | 238 lbs | R | Ohio State | Practice Squad |
| 80 | Tyree Jackson | TE | 6'7" | 249 lbs | 3 | Buffalo | Reserve |
| 80 | Colson Yankoff | TE | 6'4" | 225 lbs | 2 | UCLA | Active |
| 86 | Zach Ertz | TE | 6'5" | 250 lbs | 13 | Stanford | Reserve |
| 87 | John Bates | TE | 6'6" | 256 lbs | 5 | Boise State | Active |
| 89 | Lawrence Cager | TE | 6'5" | 220 lbs | 4 | Georgia | Practice Squad |
| 1 | Deebo Samuel Sr. | WR | 6'0" | 215 lbs | 7 | South Carolina | Active |
| 10 | Jacoby Jones | WR | 6'3" | 228 lbs | R | UCF | Practice Squad |
| 11 | Luke McCaffrey | WR | 6'2" | 195 lbs | 2 | Rice | Reserve |
| 12 | Robbie Chosen | WR | 6'3" | 185 lbs | 9 | Temple | Practice Squad |
| 13 | Treylon Burks | WR | 6'2" | 225 lbs | 4 | Arkansas | Active |
| 16 | Ja'Corey Brooks | WR | 6'3" | 195 lbs | R | Louisville | Practice Squad |
| 17 | Terry McLaurin | WR | 6'0" | 210 lbs | 7 | Ohio State | Active |
| 19 | Chris Moore | WR | 6'1" | 200 lbs | 9 | Cincinnati | Active |
| 48 | River Cracraft | WR | 6'0" | 198 lbs | 7 | Washington State | Practice Squad |
| 83 | Jaylin Lane | WR | 5'10" | 196 lbs | R | Virginia Tech | Reserve |
| 85 | Noah Brown | WR | 6'2" | 225 lbs | 9 | Ohio State | Reserve |
Defense
| # | Name | Pos | Ht | Wt | Exp | College | Status |
| — | Darius Rush | CB | 6'2" | 198 lbs | 2 | South Carolina | Practice Squad |
| 2 | Marshon Lattimore | CB | 6'0" | 192 lbs | 9 | Ohio State | Reserve |
| 6 | Noah Igbinoghene | CB | 5'11" | 197 lbs | 6 | Auburn | Active |
| 23 | Trey Amos | CB | 6'1" | 190 lbs | R | Mississippi | Reserve |
| 29 | Kevon Seymour | CB | 6'0" | 188 lbs | 7 | USC | Practice Squad |
| 31 | Jonathan Jones | CB | 5'10" | 185 lbs | 10 | Auburn | Active |
| 34 | Antonio Hamilton | CB | 6'0" | 188 lbs | 10 | South Carolina State | Active |
| — | Mike Sainristil | CB | 5'10" | 182 lbs | 2 | Michigan | Active |
| — | Tre Hawkins III | CB | 6'3" | 195 lbs | 3 | Old Dominion | Practice Squad |
| 22 | Car'lin Vigers | DB | 6'2" | 200 lbs | R | Louisiana-Monroe | Practice Squad |
| 47 | Robert McDaniel | DB | 6'2" | 207 lbs | R | Jackson State University | Practice Squad |
| — | DeMarcus Walker | DE | 6'4" | 280 lbs | 9 | Florida State | Practice Squad |
| 92 | Dorance Armstrong | DE | 6'4" | 255 lbs | 8 | Kansas | Reserve |
| — | Drake Jackson | DE | 6'4" | 273 lbs | 3 | USC | Active |
| 52 | Preston Smith | DE | 6'5" | 265 lbs | 11 | Mississippi State | Active |
| 55 | Jacob Martin | DE | 6'2" | 242 lbs | 8 | Temple | Active |
| 59 | T.J. Maguranyanga | DE | 6'4" | 222 lbs | R | — | Practice Squad |
| 90 | Javontae Jean-Baptiste | DE | 6'4" | 260 lbs | 2 | Notre Dame | Reserve |
| 91 | Deatrich Wise | DE | 6'5" | 280 lbs | 9 | Arkansas | Reserve |
| 66 | Ricky Barber | DT | 6'3" | 295 lbs | R | UCF | Practice Squad |
| 95 | Jer'Zhan Newton | DT | 6'2" | 295 lbs | 2 | Illinois | Active |
| 96 | Jalyn Holmes | DT | 6'5" | 283 lbs | 6 | Ohio State | Active |
| 98 | Sheldon Day | DT | 6'1" | 294 lbs | 9 | Notre Dame | Active |
| 99 | Javon Kinlaw | DT | 6'5" | 319 lbs | 6 | South Carolina | Active |
| — | Shy Tuttle | DT | 6'3" | 300 lbs | 7 | Tennessee | Active |
| 51 | Ale Kaho | LB | 6'2" | 230 lbs | R | UCLA | Active |
| — | DJ Johnson | LB | 6'4" | 260 lbs | 3 | Oregon | Practice Squad |
| 4 | Frankie Luvu | LB | 6'3" | 236 lbs | 8 | Washington State | Active |
| 56 | Kain Medrano | LB | 6'3" | 230 lbs | R | UCLA | Active |
| 57 | Nick Bellore | LB | 6'1" | 250 lbs | 15 | Central Michigan | Active |
| 58 | Jordan Magee | LB | 6'3" | 226 lbs | 2 | Temple | Active |
| 54 | Bobby Wagner | MLB | 6'0" | 242 lbs | 14 | Utah State | Active |
| 94 | Daron Payne | NT | 6'3" | 320 lbs | 8 | Alabama | Active |
| 97 | Eddie Goldman | NT | 6'3" | 320 lbs | 8 | Florida State | Reserve |
| 24 | Von Miller | OLB | 6'3" | 250 lbs | 15 | Texas A&M | Active |
| 3 | Will Harris | SAF | 6'1" | 207 lbs | 7 | Boston College | Active |
| 18 | Tyler Owens | SAF | 6'2" | 213 lbs | 2 | Texas Tech | Active |
| 20 | Quan Martin | SAF | 6'0" | 195 lbs | 3 | Illinois | Active |
| 35 | Percy Butler | SAF | 6'0" | 191 lbs | 4 | Louisiana-Lafayette | Active |
| 39 | Jeremy Reaves | SAF | 5'11" | 200 lbs | 6 | South Alabama | Active |
Special Teams
| # | Name | Pos | Ht | Wt | Exp | College | Status |
| 16 | Jake Moody | K | 6'1" | 210 lbs | 3 | Michigan | Active |
| 69 | Tyler Ott | LS | 6'3" | 253 lbs | 11 | Harvard | Active |
| 10 | Tress Way | P | 6'1" | 220 lbs | 12 | Oklahoma | Active |
